Magnetite nanoparticles for biosensor model based on bacteria fluorescence

A. Poita; D.-E. Creanga; A. Airinei; P. Tupu; C. Goiceanu; O. Avadanei

doi:10.2971/jeos.2009.09024

Journal of the European Optical Society - Rapid publications, Vol 4 (2009)

Magnetite nanoparticles for biosensor model based on bacteria fluorescence

A. Poita, D.-E. Creanga, A. Airinei, P. Tupu, C. Goiceanu, O. Avadanei

Abstract

Fluorescence emission of pyoverdine â€“ the siderophore synthesized by iron scavenger bacteria - was studied using in vitro cultures of Pseudomonas aeruginosa with the aim to design a biosensor system for liquid sample iron loading. Diluted suspensions of colloidal magnetite nanoparticles were supplied in the culture medium (10 microl/l and 100 microl/l) to simulate magnetic loading with iron oxides of either environmental waters or human body fluids. The electromagnetic exposure to radiofrequency waves of bacterial samples grown in the presence of magnetic nanoparticles was also carried out. Cell density diminution but fluorescence stimulation following 10 microl/l ferrofluid addition and simultaneous exposure to radiofrequency waves was evidenced. The inhibitory influence of 100 microl/l ferrofluid combined with RF exposure was evidenced by fluorescence data. Mathematical model was proposed to approach quantitatively the dynamics of cell density and fluorescence emission in relation with the consumption of magnetite nanoparticle supplied medium. The biosensor scheme was shaped based on the response to iron loading of bacterial sample fluorescence.

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References

J. M. Meyer, "Pyoverdines: pigments, siderophores and potential taxonomic markers of fluorescent Pseudomonas species" Arch. Microbiol. 174, 135-142 (2000).

T. N. Temple, V. O. Stockwell, J. Loper, and K. B. Johnson, "Bioavailability of iron to Pseudomonas fluorescens strain A506 on flowers of pear" Phytopathology 94 1286-1294, (2004).

D. C. Joyner and S. E. Lindow, "Heterogeneity of iron bioavailability on plants assessed with a whole-cell GFP-based bacterial biosensor" Microbiology+ 146 2435-2445, (2000).

M. Perry, J. McLean, A. Anderson, and C. D. Miller, "Proceedings of Subsurface Science Symposium, Boise, Idaho, 2002" Inra News, 2 (10) (2002).

M. Racuciu, D. E. Creanga, N. Sulitanu, and V. Badescu, "Dimensional analysis of aqueous magnetic fluids" Appl. Phys. A-Mater. 89 (2) (2007).

C. Goiceanu, Thesis, (University "Al. I. Cuza", Romania, 2002).

A. Manoliu, L. Oprica, and D. E. Creanga, "Ferrofluid and cellulolytic fungi" J. Magn. Magn. Mater. 289, 473-475 (2005).

S. Dunca, D. E. Creang_a, O. Ailiesei, and E. Nimitan, "Microorganisms growth with magnetic fluids" J. Magn. Magn. Mater. 289, 445-44 (2005).

J. F. B. Santana, M. A. G. Soler, S. W. da Silva, M. H. Guedes, Z. G. M. Lacava, R. B. Azevedo, and P. C. Morais, "Investigation of the interaction between magnetic nanoparticles surface-coated with carboxymethyldextran and blood cells using Raman spectroscopy" J. Magn. Magn. Mater. 289, 452-454 (2005).

M. A. Soler, S. N. Báo, G. B. Alcântara, V. H. Tibúrcio, G. R. Paludo, J. F. Santana, M. H. Guedes, E. C. Lima, Z. G. Lacava, and P. C. Morais, "Interaction of erythrocytes with magnetic nanoparticles" J. Nanosci. Nanotechno. 7 (3), 1069-1071 (2007).

R. Kumar, G. Clermont, Y. Vodovotz, and C. C. Chow, "The dynamics of acute inflammation" J. Theor. Biol. 230, 145-155 (2004).