Compact system design based on digital in-line holographic microscopy configuration

M. Mihailescu; M. Kusko

doi:10.2971/jeos.2012.12010

Journal of the European Optical Society - Rapid publications, Vol 7 (2012)

Compact system design based on digital in-line holographic microscopy configuration

M. Mihailescu, M. Kusko

Abstract

We present our study regarding a compact system design for cell counting and simultaneous 3D imaging, based on digital in-line holographic microscopy configuration. The system is built around the known experimental configuration which includes a pinhole but we also investigate the configuration with a monomode fiber as a light source. Considered samples consist of a very low concentration of cells in flow in a microchannel. The main challenge in our design is to obtain the digital hologram of one cell on a regular video camera sensor in proper resolution conditions, as opposed to the usual configurations where the aim is to visualize a large area. This fact is possible with shorter distances between pinhole and sample and with pinholes with diameters slightly larger than 1micron. These can now be realized by considering the microtechnological processes for microchannel and pinhole fabrication on the same substrate with high refractive index - to increase the numerical aperture of the system The geometrical parameters are established after the numerical analysis of the diffracted field from a single cell and of the entire system numerical aperture values.

Full Text: PDF

Citation Details

Cite this article

References

O. Mudanyali, W. Bishara, and A. Ozcan, "Lensfree superresolution holographic microscopy using wetting films on a chip," Opt. Express 19, 17378-17389 (2011).

J. P. Ryle, K. M. Molony, S. McDonnell, T. J. Naughton, and J. T. Sheridan "Multispectral lensless digital holographic microscope: imaging MCF-7 and MDA-MB-231 cancer cell cultures," Proc. of SPIE 7442, 1-11 (2009).

V. R. Singh, E. Darakis, G. Hegde and A. Asundi, "A new methodology for pixel size retention in lensless digital holographic microscopy applied to micro-particle analysis," J. Opt. 13, 035704 (2011).

T. Colomb, S. Krivec, H. Hutter, A. A. Akatay, N. Pavillon, F. Montfort, E. Cuche, J. Kühn, C. Depeursinge, and Yves Emery, "Digital holographic reflectometry," Opt. Express 18, 3719-3731 (2010).

C. Minetti, N. Callens, G. Coupier, T. Podgorski, and F. Dubois, "Fast measurements of concentration profiles inside deformable objects in microflows with reduced spatial coherence digital holography," Appl. Optics 47, 5305-5314 (2008).

D. D. Aguayo, F. M. Santoyo, M. H. De la Torre-I, M. D. Salas-Araiza, C. Caloca-Mendez, and D. A. G. Hernandez, "Insect wing deformation measurements using high speed digital holographic interferometry," Opt. Express 18, 5661-5667 (2010).

A. Ogiwara, M. Watanabe, T. Mabuchi, and F. Kobayashi, "Formation of holographic memory for defect tolerance in optically reconfigurable gate arrays," Appl. Optics 49, 4255-4261 (2010).

M. Kanka, R. Riesenberg, and H. J. Kreuzer, "Reconstruction of high-resolution holographic microscopic images," Opt. Lett. 34, 1162-1164 (2009).

E. Cuche, P. Marquet, and C. Depeursinge, "Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms," Appl. Optics 38, 6994-7001 (1999).

N. Verrier, C. Remacha, M. Brunel, D. Lebrun, and S. Coëtmellec, "Micropipe flow visualization using digital in-line holographic microscopy," Opt. Express 18, 7807-7819 (2010).

F. C. Cheong, B. Sun, R. Dreyfus, J. Amato-Grill, K. Xiao, L. Dixon and D. G. Grier, "Flow visualization and flow cytometry with holographic video microscopy," Opt. Express 17, 13071-13079 (2009).

J. F. Restrepo, and J. Garcia-Sucerquia, "Diffraction-based modeling of high-numerical-aperture in-line lensless holograms," Appl. Optics 50, 1745-1752 (2011).

S. Coëtmellec, N. Verrier, M. Brunel, and D. Lebrun, "General formulation of digital in-line holography from correlation with a chirplet function," J. Europ. Opt. Soc. Rap. Public. 5, 10027 (2010).

A. El Mallahi, and F. Dubois, "Dependency and precision of the refocusing criterion based on amplitude analysis in digital holographic microscopy," Opt. Express 19, 6684-6698 (2011).

I. Moon, F. Yi, and B. Javidi, "Automated Three-Dimensional Microbial Sensing and Recognition Using Digital Holography and Statistical Sampling," Sensors 10, 8437-8451 (2010).

M. Kanka, R. Riesenberg, P. Petruck, and C. Graulig, "High resolution (NA=0.8) in lensless in-line holographic microscopy with glass sample carriers," Opt. Lett. 36, 3651-3653 (2011).

J. Garcia-Sucerquia, W. Xu, M. H. Jericho, and H. J. Kreuzer, "Immersion digital in-line holographic microscopy," Opt. Lett. 31, 1211- 1213 (2006).

V. Mico, and Z. Zalevsky, "Superresolved digital in-line holoraphic microscopy for high-resolution lensless biological imaging," J. Biomed. Optics 15, 046027 (2010).

M. Mir, H. Ding, Z.Wang, J. Reedy, K. Tangella, and G. Popescu, "Blood screening using diffraction phase cytometry," J. Biomed. Optics 15, 027016 (2010).

P. Amsler, O. Stetzer, M. Schnaiter, E. Hesse, S. Benz, O. Moehler, and U. Lohmann, "Ice crystal habits from cloud chamber studies obtained by in-line holographic microscopy related to depolarization measurements," Appl. Optics 48, 5811-5822 (2009).

P. Ferraro, A. Wax, and Z. Zalevsky, "Coherent Light Microscopy," Chapter 1 Point Source Digital In-Line Holographic Microscopy, Springer Series in Surface Sciences 46, Springer-Verlag Berlin Heidelberg (2011).

J. W. Goodman, "Introduction to Fourier Optics," Mc Graw-Hill Book Company, (1968).

B. Rappaz, A. Barbul, Y. Emery, R. Korenstein, C. Depeursinge, P. J. Magistretti, and P. Marquet, "Comparative study of human erythrocytes by digital holographic microscopy, confocal microscopy, and impedance volume analyzer," Cytometry Part A 73, 895-903 (2008).

W. Groner, N. Mohandas, and M. Bessis "New Optical Technique for Measuring Erythrocyte Deformability with the Ektacytometer," Clin. Chem. 26 (10), 1435-1442 (1980).

F. M. Gaffney Brit, "Experimental Haemolytic Anaemia with Particular Reference to the Corpuscular Haemoglobin Concentrations of the Erythrocytes," J. Haematology 3, 311 (1957).

W. Xu, M. H. Jericho, I. A. Meinertzhagen, and H. J. Kreuzer, "Digital In-Line Holography of Microspheres," Appl. Optics 41, 5367-5375 (2002).

S. Seo, T. Su, A. Erlinger, D. Tseng, and A. Ozcan, "On-Chip Cytometry Using Lensless Digital Holography," in Conference on Lasers and Electro-Optics/International Quantum Electronics Conference, OSA Technical Digest (CD) (Optical Society of America, Washington, 2009).

J. Garcia-Sucerquia, W. Xu, S. K. Jericho, P. Klages, M. H. Jericho, and H. J. Kreuzer, "Digital in-line holographic microscopy," Appl. Optics 45, 836-850 (2006).

S. K. Jericho, P. Klages, J. Nadeau, E. M. Dumas, M. H. Jericho, and H. J. Kreuzer, "In-line digital holographic microscopy for terrestrial and exobiological research," Planet. Space Sci. 58, 701-705 (2010).

U. Schnars, and W. P. O. Juptner, "Digital recording and numerical reconstruction of holograms," Meas. Sci. Technol. 13, 85-101, (2002).

optiwave.com

L. Repetto, E. Piano, and C. Pontiggia, "Lensless digital holographic microscope with light emitting diode illumination," Opt. Lett. 29, 1132-1134 (2004).