Journal of the European Optical Society - Rapid publications, Vol 8 (2013)

Wide-field common-path incoherent correlation microscopy with a perfect overlapping of interfering beams

P. Bouchal, Z. Bouchal

Abstract


Incoherent correlation microscopy is recently discovered technique for digital imaging of three-dimensional objects in a quasimonochromatic spatially incoherent light. Its operation is based on wavefront division carried out by a spatial light modulator and capturing correlation recordings of the observed scene. To achieve image reconstruction, at least a partial overlapping of the signal and reference waves created by the spatial light modulator is necessary. In the known experimental configurations, the overlapping of interfering beams is strongly reduced in off-axis areas of the object and the image can be reconstructed only in a very small portion of the field of view provided by the used microscope objective lens. Here, we propose and successfully demonstrate modified experimental system working with two-component relay optics inserted between the microscope objective and the spatial light modulator and providing full overlapping of correlated beams in all areas of the field of view of the objective lens. The benefits and applicability of the proposed system design are clearly demonstrated on the imaging of the USAF resolution targets.

© The Authors. All rights reserved. [DOI: 10.2971/jeos.2013.13011]

Full Text: PDF

Citation Details


Cite this article

References


P. Marquet, B. Rappaz, P. J. Magistretti, E. Cuche, Y. Emery, T. Colomb, and Ch. Depeursinge, ”Digital holographic microscopy: a non invasive contrast imaging technique allowing quantitative visualization of living cells with sub wavelength accuracy,” Opt. Lett., 30(5), 468–470 (2005).

L. Lovicar, J. Komrska, and R. Chmelík, ”Quantitative-phasecontrast imaging of a two-level surface described as a 2D linear filtering process,” Opt. Express 18(20), 20585–20594 (2010)

P. Kolman, and R. Chmelík, ”Coherence-controlled holographic microscope,” Opt. Express 18(21), 21990–22003 (2010).

C. Maurer, A. Jesacher, S. Bernet, and M. Ritsch-Marte, ”What spatial light modulators can do for optical microscopy,” Laser Photonics Reviews 5, 81–101 (2011).

Z. Wang, L. Millet, M. Mir, H. Ding, S. Unarunotai, J. Rogers, M. U. Gillette, and G. Popescu, ”Spatial light interference microscopy (SLIM),” Opt. Express 19(2), 1016–1026 (2010).

Z. Wang, I. S. Chun, X. Li, Z. Y. Ong, E. Pop, L. Millet, M. Gillette, and G. Popescu, ”Topography and refractometry of nanostructures using spatial light interference microscopy,” Opt. Lett. 35(2), 208–210 (2010).

S. Furhapter, A. Jesacher, S. Bernet, M. Ritsch-Marte, ”Spiral phase contrast imaging in microscopy,” Opt. Express 13(3), 689–694 (2005).

M. Warber, S. Zwick, T. Haist, and W. Osten, ”SLM-based phasecontrast filtering for single and multiple image acquisition,” P. Soc. Photo.-Opt. Ins. 7442, 74420E–74420E-12 (2009).

M. Hasler, T. Haist, W. Osten, ”SLM-based microscopy,” P. Soc. Photo.-Opt. Ins. 8430, 84300V–84300V-8 (2012).

V. Micó, and J. García, ”Common-path phase-shifting lensless holographic microscopy,” Opt. Lett. 35(23), 3919–3921 (2010).

J. Rosen and G. Brooker, ”Fluorescence incoherent color holography,” Opt. Express 15(5), 2244–2250 (2007).

X. Lai, Y. Zhao, X. Lv, Z. Zhou, and S. Zeng, ”Fluorescence holography with improved signal-to-noise ratio by near image plane recording,” Opt. Lett. 37(13), 2445–2447 (2012).

B. Katz, J. Rosen, R. Kelner, and G. Brooker, ”Enhanced resolution and throughput of Fresnel incoherent correlation holography (FINCH) using dual diffractive lenses on a spatial light modulator (SLM),” Opt. Express 20(8), 9109–9121 (2012).

J. Rosen, N. Siegel, and G. Brooker, ”Theoretical and experimental demonstration of resolution beyond the Rayleigh limit by FINCH fluorescence microscopic imaging,” Opt. Express 19(27), 26249–26268 (2011).

P. Bouchal, and Z. Bouchal, ”Selective edge enhancement in three-dimensional vortex imaging with incoherent light,” Opt. Lett. 37(14), 2949–2951 (2012).

P. Bouchal, J. Kapitán, R. Chmelík, and Z. Bouchal, ”Point spread function and two-point resolution in Fresnel incoherent correlation holography,” Opt. Express 19(16), 15603–15620 (2011).

I. Yamaguchi and T. Zhang, ”Phase-shifting digital holography,” Opt. Lett. 22(16), 1268–1270 (1997).

L. Xu, X. Peng, Z. Guo, J. Miao, and A. Asundi, ”Imaging analysis of digital holography,” Opt. Express 13(7), 2444–2452 (2005).